小鼠 anti-人 IgE Antibody (Biotin)
ELISA, WB, FACS, Func
Monoclonal
BSW17-NBS01
IgG1 kappa
Biotin
2 images
Quick Overview for 小鼠 anti-人 IgE Antibody (Biotin) (ABIN457503)
抗原
IgE
克隆类型
单克隆
标记
Biotin
应用范围
ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
克隆位点
BSW17-NBS01
亚型
IgG1 kappa
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适用
- 人
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宿主
- 小鼠
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特异性
- Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
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灵敏度
- Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)
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产品特性
- IGHE, chromosome 14q32.33
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纯化方法
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck
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纯度
- > 85 %, tested by analytical Gel Permeation Chromatography
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过滤
- Sterile filtered
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免疫原
- purified human myeloma IgE
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Mouse anti-Human IgE (Fc Region) antibody (Biotin)
ELISA Monoclonal HP6029 IgG2a Biotin
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应用备注
- Sterile filtered solution, does not contain Na-azide. Recommended for functional cellular assays (mast cell / basophil activation).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH=7.2, sterile filtered, contains no NaN3
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储存液
- Azide free
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注意事项
- Aliquot to Avoid repeated freezing and thawing.
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储存条件
- 4 °C
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- IgE
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背景
- The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
抗原
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